Annotations: 8ZWA

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF18117	Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)	Enhanced disease susceptibility 1 protein EP domain	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].	Domain
B	PF01764	Lipase (class 3) (Lipase_3)	Lipase (class 3)	-	Family
C	PF18117	Enhanced disease susceptibility 1 protein EP domain (EDS1_EP)	Enhanced disease susceptibility 1 protein EP domain	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptor ...	The plant specific protein family, which comprises EDS1 (Enhanced disease susceptibility 1), PAD4 (Phytoalexin deficient4) and SAG101 (Senescence-associated gene 101), is involved in innate immunity [Ref.1]. Signaling by intracellular immune receptors with NB-ARC domain (Pfam:PF00931) relies on this protein family [1,3]. C-terminus of Arabidopsis EDS1 and PAD4 proteins did not have recognizable sequence homology to other domains and therefore named EP domain (EDS1-PAD4) [2]. A combination of an N-terminal lipase_3 domain (Pfam:PF01764) and the C-terminal EP-domain is the defining feature of this family. Structurally, EP domain comprises exclusively of alpha-helices (PDB:4NFU) [1]. Structure-function analysis of Arabidopsis proteins showed that EDS1 forms heterodimers with PAD4 or SAG101, chiefly through the N-terminal lipase_3 domain. These heterodimers are essential for plant defense signaling. The EP domain is not stable without the lipase_3 domain, but is required for immunity [1]. Mutations of conserved residues in the EP domain of EDS1 strongly affect its immune signaling function [4].	Domain
C	PF01764	Lipase (class 3) (Lipase_3)	Lipase (class 3)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Disease resistance protein ADR1	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ADP binding kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups	response to acid chemical response to stimulus response to stress response to chemical response to oxygen-containing compound response to abiotic stimulus response to water response to water deprivation biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus response to biotic stimulus defense response to fungus response to acid chemical response to stimulus response to stress response to chemical response to oxygen-containing compound response to abiotic stimulus response to water response to water deprivation biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus response to biotic stimulus defense response to fungus defense response to other organism response to external biotic stimulus response to fungus cellular response to stimulus immune response defense response to symbiont cell death symbiont-induced defense-related programmed cell death cellular response to stress immune system process plant-type hypersensitive response innate immune response cellular process programmed cell death defense response to bacterium response to bacterium	-
B	Isoform 1 of Protein EDS1	identical protein binding protein binding binding protein homodimerization activity protein dimerization activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity hydrolase activity catalytic activity lipase activity	developmental process anatomical structure morphogenesis aerenchyma formation anatomical structure formation involved in morphogenesis anatomical structure development response to decreased oxygen levels response to stimulus response to hypoxia response to stress response to oxygen levels response to abiotic stimulus cellular response to stimulus biological process involved in interspecies interaction between organisms response to other organism developmental process anatomical structure morphogenesis aerenchyma formation anatomical structure formation involved in morphogenesis anatomical structure development response to decreased oxygen levels response to stimulus response to hypoxia response to stress response to oxygen levels response to abiotic stimulus cellular response to stimulus biological process involved in interspecies interaction between organisms response to other organism defense response immune response defense response to symbiont response to external stimulus cell death response to biotic stimulus symbiont-induced defense-related programmed cell death cellular response to stress defense response to other organism immune system process plant-type hypersensitive response innate immune response cellular process response to external biotic stimulus programmed cell death defense response to Gram-negative bacterium defense response to bacterium response to bacterium response to oxidative stress response to singlet oxygen response to chemical response to oxygen-containing compound response to reactive oxygen species primary metabolic process metabolic process lipid metabolic process multicellular organism development multicellular organismal process leaf abscission abscission regulation of biological process regulation of cellular process regulation of hydrogen peroxide metabolic process reactive oxygen species metabolic process biological regulation hydrogen peroxide metabolic process regulation of metabolic process regulation of reactive oxygen species metabolic process signaling response to salicylic acid cell communication signal transduction salicylic acid mediated signaling pathway response to organic cyclic compound cellular response to salicylic acid stimulus systemic acquired resistance cellular response to organic cyclic compound systemic acquired resistance, salicylic acid mediated signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus	protein-containing complex EDS1 disease-resistance complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle plastid organelle intracellular membrane-bounded organelle chloroplast nucleus endoplasmic reticulum protein-containing complex EDS1 disease-resistance complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle plastid organelle intracellular membrane-bounded organelle chloroplast nucleus endoplasmic reticulum endomembrane system
C	PAD4	-	primary metabolic process metabolic process cellular process lipid metabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR039203	RGA2/3-like	Unknown
A	IPR042197	Apoptotic protease-activating factors, helical domain	Homologous Superfamily
A	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
A	IPR002182	NB-ARC	Domain
A	IPR008808	Powdery mildew resistance protein, RPW8 domain	Domain
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
B	IPR044214	EDS1-like	Family
B	IPR041266	EDS1, EP domain	Domain
B	IPR002921	Fungal lipase-type domain	Domain
C	IPR041266	EDS1, EP domain	Domain
C	IPR002921	Fungal lipase-type domain	Domain
C	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.